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Figure 4 | BMC Proceedings

Figure 4

From: Comparison of three microarray probe annotation pipelines: differences in strategies and their effect on downstream analysis

Figure 4

Ensembl Annotation Assigned to Oligos: Coverage & Consensus. Venn diagram representing oligos linked to Ensembl gene IDs by the 3 annotation pipelines (A). Colours represent oligos linked to at least one Ensembl gene by all 3 pipelines (417:black), not linked to any Ensembl genes by any of the 3 pipelines (245:white), linked to at least one Ensembl gene only by IMAD (26:red), only by OligoRAP (2:blue), only by sigReannot (13:yellow), by IMAD & OligoRAP (3:purple), OligoRAP & sigReannot (6:green) or by IMAD & sigReannot (79:orange). When an oligo is linked to at least one Ensembl gene by all 3 pipelines this not necessarily means it is linked to the same Ensembl genes, which is depicted as consensus in a pie chart (B). Agreement between all 3 pipelines is subdivided in agreement on the presence or on the absence of links to Ensembl genes. Where only 2 pipelines agree this is not subdivided and hence represents a mix of consensus on presence or absence of annotation. Pipeline's initials indicate the corresponding pipelines share consensus; a dash instead of an initial indicates the corresponding pipelines lack consensus. Reasons for a lack of consensus are sorted by impact (C) and were counted per oligo: ++ = extra hits were found because of this reason, -- = hits were missing because of this reason. If an oligo had multiple hits, multiple reasons can apply, but multiple occurrences of the same reason were counted only once.

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