Volume 4 Supplement 2

Abstracts of the 16th International Charles Heidelberger Symposium on Cancer Research

Open Access

Metabolic changes in human bronchial epithelial cells upon chronic exposure to hexavalent chromium

  • Leonardo MR Ferreira1, 2Email author,
  • Maria S Santos1, 3,
  • M Carmen Alpoim1, 3, 4 and
  • Ana M Urbano1, 2, 4
BMC Proceedings20104(Suppl 2):P16

DOI: 10.1186/1753-6561-4-S2-P16

Published: 24 September 2010

Some hexavalent chromium [Cr(VI)] compounds are well established occupational respiratory tract carcinogens. However, despite a very large number of studies, the mechanisms of Cr(VI)-induced malignization at the cellular and molecular levels are only now beginning to be understood with more detail. It has been known for decades, since the seminal studies of Otto Warburg in the 1920s, that most solid tumors exhibit a specific metabolic pattern, characterized by a strong contribution of lactic fermentation to the overall ATP production, even in the presence of ample oxygen. This particular metabolic reprogramming, known as the Warburg effect, provides the background for several diagnosis and therapeutic approaches, such as PET (positron emission tomography) and the design of inhibitors of glycolytic enzymes, respectively. Nevertheless, the exact role of the Warburg effect in carcinogenesis and, in particular, in Cr(VI)-induced lung cancer, remains elusive. In this study, the gradual changes in energy metabolism occurring during the chronic exposure of human bronchial epithelial cells, the main targets of Cr(VI)-induced carcinogenicity, to subcytotoxic or mildly cytotoxic concentrations of Cr(VI) were assessed using markers of bioenergetic status, such as glucose uptake, lactate production and adenylate energy charge. Significant changes were observed in all parameters, in a time- and dose-dependent manner, compatible with a role of the energy metabolism in the Cr(VI)-induced malignization process.



This work was partly funded by a CIMAGO grant (CIMAGO 26/07).

Authors’ Affiliations

Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade de Coimbra
Unidade de Química-Física Molecular, Faculdade de Ciências e Tecnologia, Universidade de Coimbra
Centro de Neurociências e Biologia Celular
Centro de Investigação em Meio Ambiente, Genética e Oncobiologia (CIMAGO), Faculdade de Medicina, Universidade de Coimbra


© Ferreira et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.