Volume 4 Supplement 2
Cytotoxic effect induced by combination of polyamines metabolites and endocannabinoid, anandamide, on human cancer cells: a new anticancer strategy
© Batista-de-Carvalho et al; licensee BioMed Central Ltd. 2010
Published: 24 September 2010
The sensitizing effect was also greater on multidrug-resistant cells than wild-type ones, an aspect of particular importance since conventional cancer therapy suffers from the development of drug resistance. Cell viability was determined using MTT assay . Concentrations 0-100 µM of AEA were tested, for incubation times up to 24 h; and concentrations 0-8 µM of spermine in presence of BSAO were used, for incubation times up to 1 h.
- Agostinelli E, Tempera G, Viceconte N, Saccoccio S, Battaglia V, Grancara S, Toninello A, Stevanato R: Potential anticancer application of polyamines oxidation products formed by amine oxidase: a new therapeutic approach. Amino Acids. 2010, 38: 353-368. 10.1007/s00726-009-0431-8.View ArticlePubMedGoogle Scholar
- Patsos HA, Hicks DJ, Dobson RR, Greenhough A, Woodman N, Lane JD, Williams AC, Paraskeva C: The endogenous cannabinoid, anandamide, induces cell death in colorectal carcinoma cells: a possible role for cyclooxygenase 2. Gut. 2005, 54: 1741-1750. 10.1136/gut.2005.073403.PubMed CentralView ArticlePubMedGoogle Scholar
- Supino R: MTT assays. Methods Mol Biol. 1995, 43: 137-49.PubMedGoogle Scholar
This article is published under license to BioMed Central Ltd.