Volume 4 Supplement 2

Abstracts of the 16th International Charles Heidelberger Symposium on Cancer Research

Open Access

Physiologic parameters variation in ICR mice during a chemical induced liver carcinogenesis experiment

  • Ivo Conceição-Pereira1,
  • Nuno M Paula-Santos1,
  • Filipa O Pereira1,
  • Maria J Pires1,
  • Luis F Palomino2,
  • Aura A Colaço1 and
  • Paula A Oliveira1Email author
Contributed equally
BMC Proceedings20104(Suppl 2):P33

DOI: 10.1186/1753-6561-4-S2-P33

Published: 24 September 2010

Hepatocellular carcinoma (HCC) is responsible for more then 600 000 deaths worldwide. HCC accounts for 85 to 90% of primary liver cancers [1]. Laboratory mouse is one of the best animal models to study cancer in vivo due to various features like the similarities to humans. Animal models that mimic human diseases are quite important to understand biopathology mechanisms underlying those diseases [2].

N-diethylnitrosamine (DEN) is a genotoxic carcinogen activated by a P450-dependent mono-oxigenase into a highly reactive molecule that will affect liver tissue [3].

The aim of this study was to evaluate the effect of DEN on 5 weeks old male ICR mice physiologic parameters. Mice were euthanized 7 and 14 weeks after last DEN injections. A thorough necropsy was performed and registered the weight and macroscopic evaluation of organs. Blood for hematocrit analysis was collected by cardiac puncture.

Some animals of the experimental group developed visible alterations in the liver. Significant values in biochemistry parameters between control/experimental groups were determined to alanine aminotransferase (p = 0.044) and total bilirrubin (p = 0.026).

Mean weight values were also significant between groups in the first euthanized mice (p = 0.048).


Authors’ Affiliations

CECAV, Department of Veterinary Sciences, University of Trás-os-Montes e Alto Douro
Department of Physiology, Veterinary Faculty, University of Santiago de Compostela


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© Oliveira et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.