Volume 4 Supplement 2

Abstracts of the 16th International Charles Heidelberger Symposium on Cancer Research

Open Access

The effect of cisplatin in 5637 bladder cancer cell line

  • Rosário Pinto-Leite1,
  • Regina Arantes-Rodrigues2Email author,
  • M Luís Cardoso3,
  • Lúcio Santos4, 5 and
  • Paula A Oliveira2
BMC Proceedings20104(Suppl 2):P52

DOI: 10.1186/1753-6561-4-S2-P52

Published: 24 September 2010

Cisplatin is an inorganic platinum agent (cis-diamminedichloroplatinum) with antineoplastic activity. It’s been an important component of standard treatment regimens for several human malignancies including bladder cancer [1]. Cisplatin forms highly reactive, charged, platinum complexes which bind to nucleophilic groups such as GC-rich sites in DNA, inducing intrastrand and interstrand DNA cross-links, as well as DNA-protein cross-links. These cross-links result in apoptosis and cell growth inhibition [2]. We have studied the cisplatin induced apoptotic responses of one human urinary bladder cancer cell line 5637. The cytotoxic activity of cisplatin on the survival profiles of 5637 cell line was determined by MTT assay. The survival rates of 5637 cells in the presence of 1, 2, 3, 4, and 5 μg/ml cisplatin after 72h of exposure were averaged 82.6, 55.4, 18.6, 7.2 and 7.9%, respectively. Treatment with cisplatin markedly decreased the viability of 5637 cells in a dose-dependent manner and a significant correlation was found between cell proliferation and cisplatin concentration (r = 0.964; p < 0.01).

Authors’ Affiliations

Genetics Service, Cytogenetic Laboratory, Hospital Center of Trás-os-Montes and Alto Douro
Department of Veterinary Sciences, CECAV, University of Trás-os-Montes and Alto Douro
Department of Biochemistry, Faculty of Pharmacy, University of Porto
Department of Surgical Oncology, Portuguese Institute of Oncology
Health Faculty, Fernando Pessoa University


  1. Piulats JM, Jiménez L, García del Muro X, Villanueva A, Viñals F, Germà-Lluch JR: Molecular mechanisms behind the resistance of cisplatin in germ cell tumours. Clin Transl Oncol. 2009, 11: 780-6. 10.1007/s12094-009-0446-3.View ArticlePubMedGoogle Scholar
  2. Konstantakou EG, Voutsinas GE, Karkoulis PK, Aravantinos G, Margaritis LH, Stravopodis DJ: Human bladder cancer cells undergo cisplatin-induced apoptosis that is associated with p53-dependent and p53-independent responses. Int J Oncol. 2009, 35: 401-416.PubMedGoogle Scholar


© Arantes-Rodrigues et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.