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Chimeric virus-like particles presenting common neutralizing epitopes of enterovirus 71
BMC Proceedings volume 5, Article number: P29 (2011)
To develop an effective vaccine against multiple genotypes of enterovirus 71 (EV71), we generated chimeric virus-like particles (VLPs) repetitively displaying the common neutralizing epitopes of EV71 and evaluated their immunogenicities in mice. The two conserved epitopes, encompassing amino acids 163-177 and 208-222 of VP1 of EV71, were fused to hepatitis B core antigen (HBcAg) and expressed in E.coli. The resulting fusion proteins were found to assemble into chimeric VLPs. Both unmodified HBcAg and chimeric VLPs induced HBcAg-specific antibody responses in mice, however, only chimeric VLP-immunized sera possessed EV71 epitope-specific IgG antibodies and efficiently neutralized different EV71 strains. Collectively, our results indicate that the chimeric VLP is capable of eliciting broadly neutralizing antibody responses and is therefore a promising EV71 vaccine candidate.
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This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Wang, B., Liu, Q., Shi, J. et al. Chimeric virus-like particles presenting common neutralizing epitopes of enterovirus 71. BMC Proc 5 (Suppl 1), P29 (2011). https://doi.org/10.1186/1753-6561-5-S1-P29
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DOI: https://doi.org/10.1186/1753-6561-5-S1-P29