Volume 5 Supplement 6

International Conference on Prevention & Infection Control (ICPIC 2011)

Open Access

INCF plasmids responsible by dissemination of blaKPC gene among enterobacteriaceae

  • F Calisto1,
  • L Lito2,
  • J Melo-Cristino2 and
  • A Duarte1
BMC Proceedings20115(Suppl 6):P132

DOI: 10.1186/1753-6561-5-S6-P132

Published: 29 June 2011

Introduction / objectives

In recent years, carbapenem resistance has emerged among Enterobacteriaceae in many geographical locations due to Klebsiella pneumoniae producing KPC carbapenemase. The aim of this study was to characterize the genetic elements involved in blaKPC gene mobilization and diffusion.

Methods

A total of 22 isolates, K. pneumoniae (n=20), Escherichia coli (n=1) and Enterobacter aerogenes (n=1), were collected from a university hospital, in Lisbon, Portugal. MIC were determined by Etest and interpreted according to Clinical and Laboratory Standards Institute guidelines. PCR was performed with primers designed to amplify blaSHV, blaTEM and blaKPC genes. Molecular typing was performed by M13-PCR fingerprinting and in representative strains by Multilocus Sequence Typing (MLST). Replicon typing was used to define plasmid incompatibility groups (Inc).

Results

Twenty-two isolates had MIC ranging between 2 and 32 mg/L for imipenem and meropenem. All isolates encoding KPC-3. Associated to carbapenemase 7 isolates encoded for TEM-1 or SHV-1 and 6 isolates for both TEM-1 and SHV-type. Although M13-PCR fingerprint analysis showed four predominant profiles, but with MLST only one sequence type ST11 was found among K. pneumoniae isolates. The predominant plasmid was included in IncF (90.5%) and was found plasmids belong to different replicons FIC (52.4%), HI1, HI2, and I1-Iγ (47.8%), T (42.8%), FIIAs (33.3%), W and P (32.8%) and FIB (9.5%).

Conclusion

The blaKPC gene, often associated with other β-lactamases, was transferred between K. pneumoniae, E. aerogenes and E. coli strains by IncF group plasmid. The clustering of resistance genes in plasmids and their organization with regard to cotransfer underlines the importance of these plasmids in the spread of antimicrobial multiresistance.

Disclosure of interest

None declared.

Authors’ Affiliations

(1)
i-Med, Faculdade de Farmàcia, CHLN, EPE Lisboa
(2)
Laboratory of Microbiology, CHLN, EPE Lisboa

Copyright

© Calisto et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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