Volume 5 Supplement 6

International Conference on Prevention & Infection Control (ICPIC 2011)

Open Access

Is multi-drugs resistant Acinetobacter baumannii epidemic spread related to reduced susceptibility to biocides?

  • B Casini1, 2,
  • MG Minacori1,
  • A Buzzigoli1,
  • P Valentini1, 2,
  • P Morici1,
  • S Barnini3,
  • C Tascini4,
  • F Menichetti4,
  • GM Rossolini5 and
  • G Privitera1, 2
BMC Proceedings20115(Suppl 6):P300

DOI: 10.1186/1753-6561-5-S6-P300

Published: 29 June 2011

Introduction / objectives

Aim of this study was to assess the role of active extrusion in mediating decreased susceptibility to biocides in the persistance and spread of multi-drugs resistant of A. baumannii strains isolated in intensive care units of a tertiary-care teaching hospital.

Methods

67 clinical and 24 environmental strains isolated from 2007 to 2010 were genotyped by PFGE, MLST and REP-PCR. Multiplex PCRs were performed for identification of the ompA, csuE and blaOXA-51like sequence type groups. The antimicrobial susceptibility was determined and the presence of carbapenemase-encoding genes was analysed by characterization of the blaOXA genes. Chlorine susceptibility was analysed according to BS EN 1040:1997

Results

The cross-analysis of genotyping methods allowed to group strains into 4 clones, only the clone A belonging to the Group 1 corrisponding to European II clonal complex and the clone B belonging to Group 2 clonal complex European I. Since 2008, a new variant of clone A has emerged as the predominant clinical and environmental strain, resulting positive for the presence of the carbapenemase OXA-58 plasmid-mediated and showing reduced chlorine susceptibility in vitro

Conclusion

Multi-antibiotic resistance may not be the only strategy applied by A. baumannii to spread and persist in healthcare setting, but also the increased resistance to biocides

Disclosure of interest

None declared.

Authors’ Affiliations

(1)
Dep. Experimental Pathology, MBIE, University of Pisa
(2)
U.O. Igiene ed Epidemiologia, Azienda Ospedaliero-Universitaria Pisana
(3)
U.O. Microbiologia, Azienda Ospedaliero-Universitaria Pisana
(4)
U.O. Malattie Infettive, Azienda Ospedaliero-Universitaria Pisana
(5)
Dep. Molecular Biology, University of Siena

Copyright

© Casini et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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