Effect of influenza virus infection on key metabolic enzyme activities in MDCK cells

Janke, Robert; Genzel, Yvonne; Wetzel, Maria; Reichl, Udo

doi:10.1186/1753-6561-5-S8-P129

BMC Proceedings

Table 1 Maximum enzyme activities of glycolysis, pentose phosphate pathway, TCA cycle, and glutaminolysis in MDCK cells infected with influenza A (H1N1) compared to mock-infected cells.

From: Effect of influenza virus infection on key metabolic enzyme activities in MDCK cells

			Enzyme activities in adherent MDCK cells^a (nmol/min/10⁶ cells)
	Enzyme	EC number	Mock-infected			H1N1 infected
Glycolysis
	Hexokinase	2.7.1.1	21.80	±	3.95	21.89	±	1.48
	Phosphoglucose isomerase	5.3.1.9	465.12	±	73.94	456.93	±	111.90
	Phosphofructokinase	2.7.1.11	29.28	±	6.14	30.56	±	2.08
	Fructose-1,6-bisphosphate aldolase	4.1.2.13	23.62	±	3.32	20.74	±	8.41
	Triose-phosphate isomerase	5.3.1.1	10348.06	±	1663.65	10148.62	±	698.01
	Glyceraldehyde-3-phosphate dehydrogenase	1.2.1.12	412.90	±	73.27	413.61	±	31.61
	Pyruvate kinase	2.7.1.40	1004.26	±	43.53	1001.92	±	112.95
	Lactate dehydrogenase	1.1.1.27	1266.97	±	134.69	1302.10	±	107.37
Pentose phosphate pathway
	Glucose-6-phosphate dehydrogenase	1.1.1.49	51.91	±	1.10	62.71	±	4.59	^b
	6-phosphogluconate dehydrogenase	1.1.1.44	30.61	±	5.03	38.13	±	1.05	^b
	Transketolase	2.2.1.1	18.63	±	6.65	19.19	±	2.52
	Transaldolase	2.2.1.2	23.11	±	5.84	23.23	±	6.30
Tricarboxylic acid cycle
	Pyruvate dehydrogenase	1.2.4.1	0.30	±	0.07	0.24	±	0.10
	Pyruvate carboxylase	6.4.1.1	0.48	±	0.11	0.82	±	0.23	^b
	Citrate synthase	2.3.3.1	21.55	±	1.70	25.40	±	2.84	^b
	Citrate lyase	2.3.3.8	4.48	±	0.55	6.00	±	0.98	^b
	NAD⁺-linked isocitrate dehydrogenase	1.1.1.41	0.27	±	0.02	0.34	±	0.05	^b
	NADP⁺-linked isocitrate dehydrogenase	1.1.1.42	44.27	±	2.73	44.57	±	5.37
	Fumarase	4.2.1.2	74.62	±	6.56	77.85	±	11.75
	Malate dehydrogenase	1.1.1.37	1115.42	±	90.90	1158.66	±	145.10
Glutaminolysis
	Glutaminase	3.5.1.2	3.29	±	0.29	3.90	±	0.34	^b
	Glutamine synthetase	6.3.1.2	0.68	±	0.25	0.80	±	0.25
	Glutamate dehydrogenase	1.4.1.2	2.54	±	0.51	2.20	±	0.31
	Alanine transaminase	2.6.1.2	3.21	±	0.50	3.31	±	0.03
	Aspartate transaminase	2.6.1.1	78.67	±	5.36	79.12	±	13.17
	Malic enzyme	1.1.1.40	7.61	±	0.61	9.87	±	0.62	^b
	Phosphoenolpyruvate carboxykinase	4.1.1.32	138.64	±	15.33	131.48	±	11.42
Miscellaneous
	Acetate-CoA ligase	6.2.1.1	2.01	±	0.13	2.69	±	0.34	^b

^a Mean values and 95 % confidence intervals for three biological replicates.
^b Significantly different from mock-infected cells as determined by Student's t-test (p ≤ 0.05).

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ISSN: 1753-6561

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