Cell line development using the SEFEX system

BMC Proceedings

Table 1 Cell specific productivity and product concentration obtained with fast track cell lines and optimized cell lines.

	specific productivity [pg/cell/day]		product concentration fed-batch [g/L]
Project	fast track cell line	optimised cell line	fast track cell line	optimised cell line
1 (IgG A)	11	36	0.6 ^a	1.7 ^a
2 (IgG B)	12	25	1.0 ^b	2.6 ^c
3 (IgG C)	3	18	no fed-batch	1.1 ^d

^a Fed-batch experiment during cell line development without any optimization using chemically defined basal medium and proprietary CeloFeed. ^b Fed-batch experiment after two steps of process development and scale-up to 10 L using chemically defined basal and feed medium. ^c Fed-batch experiment at 1 L bioreactor scale after two steps of optimization using chemically defined basal medium and proprietary CeloFeed. ^d Fed-batch experiment at shake flask scale using chemically defined basal medium and proprietary CeloFeed.

ISSN: 1753-6561