Table 1 Multiplication ratios of E-MSCs and BM-MSCs on various culture systems. Multiplication ratios of E-MSCs and percentage of cells in S-phase at day 5 of a 7 day run, under various modes of culture
MSC and MC screening | |||
|---|---|---|---|
BM-MSCs | Culture system | Multiplication ratio | |
T-Flasks | 0.4 ± 0.2 | ||
Cultispher-S | 0.16 ± 0.1 | ||
Cytodex-3 | -0.1 ± 0.12 | ||
Cytopore-2 | -0.5 ± 0.04 | ||
E-MSCs | T-Flasks | 2.4 ± 0.1 | |
Cultispher-S | 2 ± 0.3 | ||
Cytodex-3 | 0.7 ± 0.6 | ||
Cytopore-2 | -0.6 ± 0.3 | ||
Maximization of MSC proliferation | |||
E-MSCs on Cultispher-S | Mode of culture | Multiplication ratio | % of cells in S-phase at day 5 |
Batch | 1.5 ± 0.3 | 1.4 ± 0.3 | |
Cyclic-fed-batch | 2.6 ± 0.2 | 8 ± 1 | |
Pulsed culture | 3 ± 0.04 | 15 ± 1 | |