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Figure 1 | BMC Proceedings

Figure 1

From: Next-generation sequencing of the CHO cell transcriptome

Figure 1

Self-Self Hybridization experiment of the customized CHO microarray. The dye ratio (M-value) is plotted against the adjusted p-value (student´s t-test, FDR controlled, α=0.05). The dye ratio was calculated using the mean intensity values (A-value) of all probes belonging to one transcript from four microarray replicates. Different thresholds were used as evidence for quality: p > 0.05, M= - 1< M < 1 (pink); p ≤ 0.05, -1 < M < 1 (dark blue); p ≤ 0.05, M ≤ -1 || M ≥ 1, A < 6 (red); p ≤ 0.05, M ≤ -1 || M ≥ 1, A ≥ 6 (green); p > 0.05, M ≤ -1 || M ≥ 1 (light blue).

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