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Table 1 Standard physical process parameters for DASGIP fermentation

From: Improving volumetric productivity of a stable human CAP cell line by bioprocess optimization

process parameter

 

T

37°C

V

0.6L

stirring

marine impeller, 120rpm

aeration

sparging, 0.03vvm

pH

7.0 (CO2/ 0.5M NaOH)

DO

40% (air saturation)

starting cell density

3-5E5mL-1