Volume 5 Supplement 8
The neuroprotective effects of electrolyzed reduced water and its model water containing molecular hydrogen and Pt nanoparticles
© Yan et al; licensee BioMed Central Ltd. 2011
Published: 22 November 2011
Characteristics of the water samples.The characteristics of water samples were determined immediately after the preparation of ERW. ERW, electrolyzed reduced water; CW, activated charcoal-treated water. The pH values were shown as average ± standard deviation (N = 5). The values of DH, DO and Pt NPs were shown the minimum and maximum values after 5 independent measurements.
11.3 ± 0.1
11.6 ± 0.1
7.9 ± 0.1
9.6 ± 0.2
Dissolved Hydrogen (mM)
Dissolved Oxygen (μM)
Pt NPs (nM)
Redox potential value (mV)
Materials and methods
In this research, we used TI-200S ERW derived from 2 mM NaOH solution produced by a batch type electrolysis device and model waters containing molecular hydrogen and synthetic Pt NPs of 2-3 nm sizes as research models of ERW to examine the anti-oxidant capabilities of ERW on several kinds of neural cells such as PC12, N1E115, and serum free mouse embryo (SFME) cells. We pretreated the ERW and 200 μM H2O2 and examined the neuroprotective effects of ERW on PC12, N1E115 and SFME cells, using WST-8 method. We also examined the intracellular ROS scavenging effects of ERW on N1E115 cells after pretreated cells with ERW and H2O2 using DCFH-DA. We checked the protective effects of ERW on mitochondria and cytoplasm by Rh123 and Fuo-3 AM stain. We also examined the ATP production of SFME cells after pretreated with ERW and H2O2 by Bioluminescence Assay Kit. Finally, we used dissolved hydrogen (DH) and Pt NPs as research models to examine their neuroprotective effects.
The results suggest that ERW is beneficial for the prevention and alleviation of oxidative stress-induced human neurodegenerative diseases.
- Shirahata S, Kabayama S, Nakano M, Miura T, Kusumoto K, Gotoh M, Hayashi H, Otsubo K, Morisawa S, Katakura Y: Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage. Biophys Biochem Res Commun. 1997, 234: 269-274. 10.1006/bbrc.1997.6622.View ArticleGoogle Scholar
- Yan H, Tian H, Kinjo T, Hamasaki T, Tomimatsu K, Nakamichi N, Teruya K, Kabayama S, Shirahata S: Extension of the lifespan of Caenorhabditis elegans by the use of electrolyzed reduced water. Biosci Biotech Biochem. 2010, 74: 2011-2015. 10.1271/bbb.100250.View ArticleGoogle Scholar
- Ohsawa I, Ishikawa M, Takahashi K, Watanabe M, Nishimaki K, Yamagata K, Katsura K, Katayama Y, Asoh S, Ohta S: Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radials. Nature Med. 2007, 13: 688-694. 10.1038/nm1577.View ArticlePubMedGoogle Scholar
- Hamasaki T, Kashiwagi T, Imada T, Nakamichi N, Aramaki S, Toh K, Morisawa S, Shimakoshi H, Hisaeda Y, Shirahata S: Kinetic analysis of superoxide anion radical-scavenging and hydroxyl radical-scavenging activities of platinum nanoparticles. Langmuir. 2008, 24: 7354-7364. 10.1021/la704046f.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.