A two step Bayesian approach for genomic prediction of breeding values
© Shariati et al.; licensee BioMed Central Ltd. 2012
Published: 21 May 2012
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© Shariati et al.; licensee BioMed Central Ltd. 2012
Published: 21 May 2012
In genomic models that assign an individual variance to each marker, the contribution of one marker to the posterior distribution of the marker variance is only one degree of freedom (df), which introduces many variance parameters with only little information per variance parameter. A better alternative could be to form clusters of markers with similar effects where markers in a cluster have a common variance. Therefore, the influence of each marker group of size p on the posterior distribution of the marker variances will be p df.
The simulated data from the 15th QTL-MAS workshop were analyzed such that SNP markers were ranked based on their effects and markers with similar estimated effects were grouped together. In step 1, all markers with minor allele frequency more than 0.01 were included in a SNP-BLUP prediction model. In step 2, markers were ranked based on their estimated variance on the trait in step 1 and each 150 markers were assigned to one group with a common variance. In further analyses, subsets of 1500 and 450 markers with largest effects in step 2 were kept in the prediction model.
Grouping markers outperformed SNP-BLUP model in terms of accuracy of predicted breeding values. However, the accuracies of predicted breeding values were lower than Bayesian methods with marker specific variances.
Grouping markers is less flexible than allowing each marker to have a specific marker variance but, by grouping, the power to estimate marker variances increases. A prior knowledge of the genetic architecture of the trait is necessary for clustering markers and appropriate prior parameterization.
The statistical methods for genomic selection introduced by Meuwissen et al ; i.e. SNP-BLUP, BayesA and BayesB, are still the most popular ones. G-BLUP that exploits SNP genotypes to build genomic relationship matrix emerged some years later and it was shown that G-BLUP and SNP-BLUP are equivalent models [2, 3]. From other approaches for genomic prediction of breeding values (BV) one can name LASSO , Bayesian LASSO  and reproducing Hilbert space models . These latter methods have not led to magnificent improvement over the original methods proposed by Meuwissen et al.  in BV prediction.
In BayesA and BayesB the prior distribution of each marker effect is assumed normal with a marker specific variance . The marker variances follow a scaled-inverse Chi-squared distribution with some scale and degrees of freedom (df) hyperparameters, a priori . Therefore, the marginal posterior distribution of marker effects is a t-distribution . In this setting, the resulted drawback is that the contribution of each marker to the posterior distribution of marker variances is only one df, that is very little information for any prior specification . As a remedy, Gianola et al  suggested to form clusters of markers where markers in a cluster have a common variance. Therefore, the influence of each marker group of size p on the posterior distribution of the marker variances will be p df. Further, they suggested assigning noninformative priors to scale and df parameters of the distribution of marker variance or marker-group variance. In the present study, a strategy for grouping markers with similar effects together was applied to the simulated data from the 15th QTL-MAS workshop . The analysis was performed in two steps, where, in the first step marker effects were estimated in a SNP-BLUP analysis, and in the next step, markers with similar effects were allocated to one group. From complexity point of view, this strategy stands between SNP-BLUP where a single variance is common to all markers, and BayesA-B, where each marker has a variance. The aim of this study was to investigate the performance of this strategy on the accuracy of genomic breeding values. Further, the effect of prior setting for the marker-group variances on the extent of phenotypic variance explained by each group of markers was investigated.
First, an animal model BLUP using pedigree and phenotypes was performed to predict breeding values of all animals, and REML estimate of heritability was obtained . Further, the heritability and breeding values were estimated using genomic models  and compared with the ones from animal model.
where μ is the general mean, β j is the allele substitution effect of the jth SNP with , x ij is the genotype covariate of the jth marker for the ith animal, associating marker effects β j to the phenotype y i , and e i is residual or environmental effect with . The genotype covariate is initially coded as 0,1,2 for homozygote, heterozygote and alternate homozygote, and then centered to have mean zero. Flat priors were considered for the residual variance and marker variance to estimate them in a reference Bayesian approach that uses the frequentist likelihood as the Bayesian posterior distribution.
where β jk denotes the effect of the jth marker that belong to group k, and g is the total number of groups. A model was used with a variance parameter per group, and the group variances are jointly modeled to have an inverse chi-square distribution in which the scale is treated as a model parameter. The prior specification was as follows:
μ~uniform; ; ; ; ; scale~uniform(>0); and df: a fixed number as hyperparameter.
In further analyses, respectively, 1500 (SNP1500) and 450 (SNP450a, SNP450b) markers with the largest effects from model (2) were selected and were allocated to groups of size 150 (for 1500 markers), and 75 or 50 (for 450 markers). Then, breeding values of animals without records were predicted using the marker effects from these subsets of markers.
Gibbs sampling was used to sample from joint posterior distributions for all datasets. The chain length was 50.000 in all analyses where the first 20.000 samples were discarded as burn in and one of each 30 samples were saved to compute the posterior means for the parameters. Preliminary experience showed that a burn in of size 20.000 guarantees the convergence for different parameters.
The challenge was to predict the breeding values of 1000 genotyped animals with no phenotypes. The available data comprised of 2000 animals with both genotype and phenotype. We validated the models using 200 animals such that the last progeny with genotype and phenotype record from each dam was taken out of the data and used for validation. The remaining 1800 animals were used to train the model. Based on this validation the size of the SNP-groups was chosen to be 150 and the scale and df of the prior distribution of marker variances were set to zero because this resulted in the highest accuracy for the validation animals. A scale and df setting of zero corresponds to the so-called Jeffreys or non-informative prior for variances. After the true breeding values of the other 1000 animals were provided, it turned out that other prior specification for the marker variances can give better predictive abilities. Perhaps, the reason was that the 200 validation animals were not enough to represent the whole population. Further, there was an imprinted QTL where the effect is expressed if it has been transmitted from one of the parents only. It is likely that among these 200 animals most of them or all of them have got the paternal (maternal) imprinted QTL. Further details of the impact of prior specification of the marker variances on estimation of SNP effects and breeding values will be discussed.
Correlation between predicted breeding values of unphenotyped animals and their true genetic values or expected genetic values of their progeny
Estimates of heritability from different methods
Grouping markers is less flexible than allowing each marker to have a specific marker variance but, by grouping, the power to estimate marker variances increases. A prior knowledge of the genetic architecture of the trait is necessary in order to clustering markers and appropriate prior parameterization. In the workshop data set, the presented approach to group SNPs gave better predictions than a SNP-BLUP model, but worse predictions than a mixture (BayesB type) model. However, the workshop data set had a limited amount of QTL, which may not be representative for many real data sets. In real data often little advantages are seen for mixture models compared to SNP-BLUP, and as our method clearly outperformed SNP-BLUP our method could be of interest for further study in real data.
MMS was funded by The Danish Research Agency grant no. 274-08-0068.
This article has been published as part of BMC Proceedings Volume 6 Supplement 2, 2012: Proceedings of the 15th European workshop on QTL mapping and marker assisted selection (QTL-MAS). The full contents of the supplement are available online at http://www.biomedcentral.com/bmcproc/supplements/6/S2.
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