Volume 7 Supplement 2

São Paulo Advanced School of Comparative Oncology: Abstracts

Open Access

Genotype and phenotype of balb/c mouse strain expressing h-2kb-tsa58- sv40 immortalizing oncogene

  • Aline C Custódio1Email author,
  • Fabiane CF Brito1,
  • Mara S Junqueira2,
  • Roger Chammas2 and
  • José E Belizário1
BMC Proceedings20137(Suppl 2):P3

DOI: 10.1186/1753-6561-7-S2-P3

Published: 4 April 2013

Introduction

The Simian Virus 40 (SV40) large T antigen is multifunctional protein with DNA helicase, RNA helicase and ATPse activities which contribute to multistep tumorogenesis in rodents and humans. The Immortomouse mouse strain expresses a mutated large T antigen tsA58 oncogene under the control of the interferon inducible murine H-2Kb promoter on chromosome 16. Our aim was to establish a BALB/c strain of H-2Kb-tsA58 immortomice that could be utilized to investigate specific pathological and physiological patterns associated SV-40 oncogenicity and generation of conditionally immortal cells lines.

Methods and results

We have crossed H2Kb-SV40-tsA58 CBA/CaxC57BL/10 hybrid immortomice with BALB/c mice to obtain a transgenic colony with unique BALB/c background. We have used two pre-validated PCR genotype assays that can distinguish between wild-type, hemizygous, and homozygous animals (4-6 months old). Enlargement of thymus is a phenotypic abnormality of immortomouse. We have characterized macroscopically and by immunohistochemistry in the F1-3 offspring hemizygous females with thymic hyperplasia (2:5 ratio). Studies are underway to typing T cell (CD4/8) populations in the thymuses. Moreover, we observed four small males displaying abnormality after birth.

Conclusion

This transgenic mouse strain will help to isolate immortalizing cell lines growing under the permissive 33°C temperature for the studies of the SV40 large T antigen transformation.

Financial support

CNPq, ICGEB.

Authors’ Affiliations

(1)
Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo
(2)
Department of Radiology and Oncology, Medical School of University of São Paulo

Copyright

© Custódio et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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