Table 1 Summary of results of virus production processes tested in various cell lines in iCELLis bioreactors (or predecessors).
Cells | Virus | Bioreactor | Surface Area (m2) | Average Cell Density at TOI (cells/cm2) | Specific Virus Productivity | Total Virus | ||
|---|---|---|---|---|---|---|---|---|
CEF | Modified Vaccina Ankara | Artefix | 0.07 | 3.9E+05 | 3.0E+06 | pfu/cm2 | 2.1E+09 | pfu |
MDBK | Bovine Herpes Virus | iCELLis nano | 4 | 1.2E+05 | 2.2E+07 | pfu/cm2 | 8.7E+11 | pfu |
iCELLis pilot | 20 | 1.4E+05 | 1.7E+07 | pfu/cm2 | 3.4E+12 | pfu | ||
iCELLis 500 | 66 | 3.3E+05 | 3.3E+07 | pfu/cm2 | 2.2E+13 | pfu | ||
A549 | rAAV | iCeLLis nano | 0.53 | 6.0E+04 | 5.3E+08 | vg/cm2 | 2.8E+12 | vg |
Adenovirus | iCELLis nano | 2.67 | 2.3E+05 | 1.1E+10 | TCID50/cm2 | 3.0E+14 | TCID50 | |
Vero | Influenza | iCELLis nano | 4 | 1.0E+05 | 3.8E+06 | TCID50/cm2 | 1.5E+11 | TCID50 |
iCELLis pilot | 20 | 7.5E+04 | 2.5E+06 | TCID50/cm2 | 5.0E+11 | TCID50 | ||
Paramyxovirus | iCELLis nano | 2.67 | 2.7E+05 | 6.4E+05 | TCID50/cm2 | 1.7E+10 | pfu | |
Vero | Undisclosed Lytic Virus | iCELLis pilot | 40 | 1.5E+05 | Confidential | Confidential | ||
iCELLis 500 | 133 | 1.5E+05 | ||||||
iCELLis 1000 | 660 | 2.3E+05 | ||||||