Figure 1
From: Evaluation of the impact of matrix stiffness on encapsulated HepaRG spheroids
Characterization of encapsulated cultures of HepaRG spheroids (A) Viability assessed by staining the encapsulated spheroids with fluorescein diacetate (live, green) and TO-PRO3 ® (dead, red). Spheroids in 1.1 and 2% (w/v) of alginate after 14 days of culture are represented. Scale bar: 100 μm (B) Growth profile of encapsulated cultures of 1.1 and 2% (w/v) of alginate. (C) Immunofluorescence characterization of hepatic spheroids (1.1% alginate) after 14 days of culture. Actin filaments - green; Nuclei - blue. Scale bar: 10 μm.