Table 1 Formation of different HMW species using different induction methods.
From: Characterization of mAb aggregates in a mammalian cell culture production process
Induction Method | Small aggregates | Large aggregates | |
|---|---|---|---|
| Â | Dimer | Oligomer | Â |
Heat | - | - | Up to 1 μm |
pH | Increase with pH | + | At pH 5 and 6 |
NaCl | Increase with NaCl | - | Above 500 mM |
Freeze-thawing | + | + | - |