Parameters | Media screen for GFP transfection | Optimize of GFP transfection | Comparison of transfection method | |
---|---|---|---|---|
Media type | ProCHO5, Opti-MEM, CHOMACS CD, ProCHO4, UltraCHO, DMEM/F12 | Opti-MEM + CHOMACS CD | Opti-MEM + CHOMACS CD | |
Transfection volume (w/o or w media addition at 5 hpt*) | 5 mL | 12.5 + 12.5 mL | 2.5 + 2.5 mL | |
Transfection protocol | Insitu | Insitu | Conventional | Insitu |
Cells amount | 10 x 106 | 62 x 106 | 10 x 106 | |
pEGFP-N1 plasmid pDNA (μg) | 6 | 37.5 | 5 | 6 |
Linear PEI 25 Kda (μg) | 30 | 187.5 | 15 | 30 |
Scale | Spin tube®50 bioreactor | 25 mL-Shake flask | Spin tube ®50 bioreactor |