Figure 3
From: Characterization of the human Nα-terminal acetyltransferase B enzymatic complex
hNatB purification and enzymatic activity. A. Coimmunoprecipitation of GST-hNaa25p and hNaa20p-CTAP or GST-hNaa25p and hNaa20p. B. NAT activity of immunoprecipitated hNatB complexes from Hela cells overexpressing GST-hNaa25p and hNaa20p-CTAP with anti-hNaa20p or anti-GST antibodies. Human tropomyosin-1 or p53 aminoterminal peptides synthesized by Dr Francisco Borrás-Cuesta at CIMA University of Navarra as previously described [27] were used as substrates. The results are representative of three independent experiments.