PEPTIDE | CPM |
---|
No-peptide
| 219 |
Tropomyosin-1
| 5290 |
Tropomyosin-1 MA
| 283 |
Tropomyosin-1 AD
| 1724 |
Tropomyosin-1 AA
| 221 |
β-actin
| 4022 |
β-actin MA
| 3760 |
β-actin AD
| 2678 |
β-actin AA
| 2951 |
- To determine the relevance of the first two amino acids there were used tropomyosin-1 and β-actin aminoterminal peptides (20 amino acids long) as well as modifications of these peptides with the first (AD), second (MA) or both (AA) amino acids changed to an alanine in a NAT assay with immunopurified hNatB complexes. The results are representative of at least two independent experiments.