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Figure 4 | BMC Proceedings

Figure 4

From: Application of reverse-phase HPLC to quantify oligopeptide acetylation eliminates interference from unspecific acetyl CoA hydrolysis

Figure 4

Standard curves of increasing amount of the substrate 1 MLGP-RRR 24 and acetyl CoA analysed by reverse phase HPLC. A; Five different amounts of the oligopeptide 1MLGP-RRR24, diluted in acetylation buffer, were analysed by reverse phase HPLC. The resulting absorption signals at 215 nm were quantified. Each amount were analysed three times and error bars indicate S.D. B; Five different amounts of acetyl CoA, diluted in acetylation buffer, were analysed by reverse phase HPLC. The resulting absorption signals at 260 nm were quantified. Each amount were analysed three times and error bars indicate S.D.

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