Figure 5
The radioactive signal from oligopeptides being modified with radioactive acetyl CoA. The oligopeptide 1MLGTE-RRR24 (200 μM) was incubated with [1-14C] acetyl CoA (final 300 μM with specific activity 11.2 mCi/mmol) and purified MBP-hNaa30p (80 nM) in acetylation buffer for 60 minutes at 37°C. Samples were collected at indicated time points and analysed with reverse phase HPLC that had been connected to a radioactivity flow detector after the absorbance detector. A clear time dependent increase in the radioactivity signal is observed, verifying that the eluted oligopeptides are labelled with radioactive acetyl groups.