Determination of Km and Vmax for the
oligopeptide, based on the generation of CoA. Purified MBP-hNaa30p (80 nM) was incubated with 300 μM acetyl CoA and varying concentrations of 1MLGTG-RRR24 (30 – 350 μM) in acetylation buffer for 30 minutes at 37°C. A; Non linear regression analysis of the dose dependent curve generated based on the analysis of the CoA absorption signal at 260 nm. The coefficient of determination (R2) is given above the plot. B; Hanes-Woolf plot of the dose dependent acetylation signal.