Table 1 Primers used in Pyrosequencing and quantitative PCR
Genes | Primers | Sequence | Purpose |
|---|---|---|---|
CD4 | F R Sequencing Assay | 5’- TTGAGATTATAYGTATTTGGAAGA -3’ 5’- GGGACACCGCTGATCGTTTA ACCTTTATATCTCCTCCTCTCCA -3’ 5’- AGTATTTATTGAGAGAAGTT -3’ 5’- Y GTAGATTGTAGTAGAGTTTGGAT Y G GTAGTAAGAT Y GTGTTGA Y GTTTT -3’ | Pyrosequencing |
GAPDH | F R | 5’-GAGGGTAGTGAAGGCTGCTG-3’ 5’-ACCAGGAAACAAGCTTGACG-3’ | quantitative PCR |
CD4 | F R | 5’- TGTCAACGCCGGATGTATAA-3’ 5’- CTTGTCCATTGGCTCCTCTC-3’ | quantitative PCR |