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Table 1 Levels of luciferase yield in the medium of CHO cells transfected with plasmids encoding Oik1 SP wild-type and 20 Oik1 SP mutants randomised in positions 4, 5 and/or 13 (mutated residues underlined), and hydropathy scores of the AAs in the respective SPs.

From: Enhanced protein synthesis and secretion using a rational signal-peptide library approach as a tailored tool

SP AA sequence Protein yield in mediuma) Hydropathy scoreb)
Wild-type MLLLSALLLGLAHGYS 100 100
Mutant 1 MLLLSALLLGLAFGYS 246 139
Mutant 2 MLLLSALLLGLACGYS 224 117
Mutant 3 MLLSFALLLGLALGYS 222 139
Mutant 4 MLLVLALLLGLALGYS 201 167
Mutant 5 MLLLSALLLGLAMGYS 191 126
Mutant 6 MLLRIALLLGLAHGYS 188 49
Mutant 7 MLLLSALLLGLAIGYS 185 144
Mutant 8 MLLLPALLLGLAHGYS 142 107
Mutant 9 MLLVPALLLGLAHGYS 126 108
Mutant 10 MLLQYALLLGLAHGYS 89 64
Mutant 11 MLLLSALLLGLAPGYS 89 113
Mutant 12 MLLLSALLLGLARGYS 82 47
Mutant 13 MLLKVALLLGLAAGYS 77 94
Mutant 14 MLLRTALLLGLAWGYS 74 43
Mutant 15 MLLKVALLLGLAHGYS 70 69
Mutant 16 MLLLSALLLGLAAGYS 70 125
Mutant 17 MLLFSALLLGLAHGYS 63 103
Mutant 18 MLLVQALLLGLAHGYS 63 84
Mutant 19 MLLRGALLLGLAVGYS 12 64
Mutant 20 MLLEHALLLGLAHGYS 0 50
  1. a) Average protein yield obtained from 3 independent transient transfections, given in percent with respect to the value obtained with Oik1 wild-type set to 100%.
  2. b) Sum of hydropathy scores of AAs at positions 4-13, judged to comprise the h-region of the Oik1 SP/SP mutants, given in percent with respect to the score for Oik1 wild-type set to 100%.