Enhanced protein synthesis and secretion using a rational signal-peptide library approach as a tailored tool

BMC Proceedings

Table 1 Levels of luciferase yield in the medium of CHO cells transfected with plasmids encoding Oik1 SP wild-type and 20 Oik1 SP mutants randomised in positions 4, 5 and/or 13 (mutated residues underlined), and hydropathy scores of the AAs in the respective SPs.

SP	AA sequence	Protein yield in medium^a)	Hydropathy score^b)
Wild-type	MLLLSALLLGLAHGYS	100	100
Mutant 1	MLLLSALLLGLAFGYS	246	139
Mutant 2	MLLLSALLLGLACGYS	224	117
Mutant 3	MLLSFALLLGLALGYS	222	139
Mutant 4	MLLVLALLLGLALGYS	201	167
Mutant 5	MLLLSALLLGLAMGYS	191	126
Mutant 6	MLLRIALLLGLAHGYS	188	49
Mutant 7	MLLLSALLLGLAIGYS	185	144
Mutant 8	MLLLPALLLGLAHGYS	142	107
Mutant 9	MLLVPALLLGLAHGYS	126	108
Mutant 10	MLLQYALLLGLAHGYS	89	64
Mutant 11	MLLLSALLLGLAPGYS	89	113
Mutant 12	MLLLSALLLGLARGYS	82	47
Mutant 13	MLLKVALLLGLAAGYS	77	94
Mutant 14	MLLRTALLLGLAWGYS	74	43
Mutant 15	MLLKVALLLGLAHGYS	70	69
Mutant 16	MLLLSALLLGLAAGYS	70	125
Mutant 17	MLLFSALLLGLAHGYS	63	103
Mutant 18	MLLVQALLLGLAHGYS	63	84
Mutant 19	MLLRGALLLGLAVGYS	12	64
Mutant 20	MLLEHALLLGLAHGYS	0	50

^a) Average protein yield obtained from 3 independent transient transfections, given in percent with respect to the value obtained with Oik1 wild-type set to 100%.
^b) Sum of hydropathy scores of AAs at positions 4-13, judged to comprise the h-region of the Oik1 SP/SP mutants, given in percent with respect to the score for Oik1 wild-type set to 100%.

ISSN: 1753-6561