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Table 1 Process optimization.

From: Use of Micro Bioreactor systems to streamline cell line evaluation and upstream process development for monoclonal antibody production

Table 1A – Medium Development

Medium

Shake flasks (120mL)

Bioreactor (2000mL)

Micro-24 (7mL)

Medium 1

100%

100%

100%

Medium 2

129%

125%

124%

Medium 3

121%

131%

140%

Medium 4

153%

175%

159%

Table 1B – Condition Optimisation

 

pH

Temperature

Relative titre

Condition 1

High

Low

58%

Condition 2

High

High

100%

Condition 3

Low

Low

96%

Condition 4

Low

High

141%