Skip to main content

Advertisement

Table 1 Analysis of the stability or recombinant protein expression in 5 independent pools of CHO cells.

From: Rapid recombinant protein production from pools of transposon-generated CHO cells

CHO-Pool Days post transfection % eGFP positive cells (*) TNFR:Fc productivity (mg/L) (°)
  30 92.8 ± 2.1 430 ± 46
1 60 93.3 ± 0.2 455 ±10
  90 92.5 ± 1.2 473 ± 10
  30 90.0 ± 0.8 348 ± 36
2 60 91.4 ± 0.5 370 ± 8
  90 94.0 ± 1.1 325 ± 35
  30 94.0 ± 1.5 432 ± 25
3 60 94.2 ± 1.1 451 ± 28
  90 91.9 ± 1.8 494 ± 16
  30 92.0 ± 0.2 432 ± 30
4 60 90.1 ± 2.0 407 ± 23
  90 92.3 ± 1.3 406 ± 28
  30 96.1 ± 1.5 388 ± 52
5 60 92.4 ± 1.4 466 ± 19
  90 93.6 ± 0.2 372 ± 12
  1. (*) determined by GUAVA flow cytometry; (°) determined by ELISA.