Table 1 Analysis of the stability or recombinant protein expression in 5 independent pools of CHO cells.
From: Rapid recombinant protein production from pools of transposon-generated CHO cells
CHO-Pool | Days post transfection | % eGFP positive cells (*) | TNFR:Fc productivity (mg/L) (°) |
|---|---|---|---|
30 | 92.8 ± 2.1 | 430 ± 46 | |
1 | 60 | 93.3 ± 0.2 | 455 ±10 |
90 | 92.5 ± 1.2 | 473 ± 10 | |
30 | 90.0 ± 0.8 | 348 ± 36 | |
2 | 60 | 91.4 ± 0.5 | 370 ± 8 |
90 | 94.0 ± 1.1 | 325 ± 35 | |
30 | 94.0 ± 1.5 | 432 ± 25 | |
3 | 60 | 94.2 ± 1.1 | 451 ± 28 |
90 | 91.9 ± 1.8 | 494 ± 16 | |
30 | 92.0 ± 0.2 | 432 ± 30 | |
4 | 60 | 90.1 ± 2.0 | 407 ± 23 |
90 | 92.3 ± 1.3 | 406 ± 28 | |
30 | 96.1 ± 1.5 | 388 ± 52 | |
5 | 60 | 92.4 ± 1.4 | 466 ± 19 |
90 | 93.6 ± 0.2 | 372 ± 12 |