Table 1 eGFP expression level of different recombinant cell lines according to puromycin concentration.
Recombinant cell line | Puromycyn (µg/ml) | Single-step selection protocol | Multistep gradual selection protocol |
|---|---|---|---|
| Â | Â | Fold increase eGFP expression (X-mean) | |
CL1 | 1 | 1.0 | 1.0 |
CL5 | 5 | 2.0 | 2.0 |
CL10 | 10 | 2.0 | 2.0 |
CL50 | 50 | 2.0 | 2.0 |
CL100 | 100 | nv | 4.5 |
CL150 | 150 | nv | 4.5 |
CL200 | 200 | nv | 6.0 |
CL250 | 250 | nv | nv |