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Table 1 Intracellular rht-PA content (% of control) on CHO cells by inhibition of translation and glycosylation prosesses and ERAD I and II pathways at 37°C and 33°C.

From: Protein folding and glycosylation process are influenced by mild hypothermia in batch culture and by specific growth rate in continuous cultures of CHO cells producing rht-PA

    

Dilution rate (h-1)

    

0.014

0.012

 

Temperature

 

Temperature

Temperature

Batch Cultures

37°C

33°C

Continuous Cultures

37°C

33°C

37°C

33°C

CC

1001

1002

SS

1003

1004

1005

1006

TM*

107

140

CHX/ERAD I**

120

117

185

139

TM/ERAD I*

87

201

CHX/ERAD II**

107

115

242

150

TM/ERAD II*

79

176

     
  1. CC: Control Culture; TM: Culture inhibited glycosylation; TM/ERAD I or II: Culture inhibited glycosylation and ERAD I or II; SS: Steady State; CHX/ERAD I or II: Culture inhibited translation and ERAD I or II; *Values at 24 hours after perturbation with inhibitors respect to CC value at 0 h. **At 48 hours after perturbation with inhibitors respect to value at SS.
  2. 1Concentration (8,8 ng/106 cells).
  3. 2Concentration (8,6 ng/106 cells).
  4. 3Concentration (7,9 ng/106 cells).
  5. 4Concentration (6,5 ng/106 cells).
  6. 5Concentration (4,2 ng/106 cells).
  7. 6Concentration (6,1 ng/106 cells).
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BMC Proceedings

ISSN: 1753-6561

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