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Figure 1 | BMC Proceedings

Figure 1

From: Differential affects of low glucose on the macroheterogeneity and microheterogeneity of glycosylation in CHO-EG2 camelid monoclonal antibodies

Figure 1

The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody. A. Lipid-linked oligosaccharide (LLO) profiles. The glycans from each sample were acid hydrolyzed from the lipid carriers, 2-AB labeled and detected by HILIC. (Glc Δ Man Ο and GlcNAc ?). B. Separation of EG2 antibodies on reduced 8-16% SDS-PAGE gel. The purified antibody in lane 8 was isolated from the culture prior to the 24 h incubation. Upper bands in lanes 1 to 4 correspond to glycosylated antibodies, and the lower bands were determined to be non-glycosylated antibodies. C. HPLC profiles of N-glycans isolated from EG2 antibodies produced by CHO cells with various initial glucose concentrations during a 24 h incubation. D. The effect of exposure time of cells to media depleted of glucose on the galactosylation (GI; ¦) and the sialylation (SI; ?) indices of EG2 antibodies produced by CHO cells.

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BMC Proceedings

ISSN: 1753-6561

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