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Table 1 Feeding regimens in fed-batch cultures.

From: Benchmarking of commercially available CHO cell culture media for antibody production

Basal medium

ActiCHO Feed A

ActiCHO Feed B

EfficientFeed A

FunctionMAX

Feed 1

Peak cell conc. [106 c/ml]

Harvest Titer [g/L]

ActiCHO P

daily; 3%

daily; 0.3%

-

-

 

23.9

5.48

ActiCHO P

daily; 3%

daily; 0.3%

-

3, 5, 7; 3.3%

 

21.3

5.82

CD OptiCHO

-

-

3, 5, 7, 9; 10%

-

 

5.8

0.72

CD OptiCHO

-

-

3, 5, 7; 10%

-

 

5.2

0.80

CD OptiCHO

-

-

3, 5, 7; 10%

3, 5, 7; 3.3%

 

6.3

1.74

CD OptiCHO

daily; 3%

daily; 0.3%

-

-

 

9.0

1.46

BalanCD CHO

-

-

-

-

1, 3, 5; 10%

7.1

1.30

  1. The time [d] for feed addition and the feed volume in % of the culture volume are indicated. Feed start for the culture in BalanCD CHO was day 1, all other cultures were fed from day 3 on. Values for peak cell concentration and harvest titer are mean values of triplicate experiments.
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BMC Proceedings

ISSN: 1753-6561

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