Skip to main content

Table 1 Feeding regimens in fed-batch cultures.

From: Benchmarking of commercially available CHO cell culture media for antibody production

Basal medium ActiCHO Feed A ActiCHO Feed B EfficientFeed A FunctionMAX Feed 1 Peak cell conc. [106 c/ml] Harvest Titer [g/L]
ActiCHO P daily; 3% daily; 0.3% - -   23.9 5.48
ActiCHO P daily; 3% daily; 0.3% - 3, 5, 7; 3.3%   21.3 5.82
CD OptiCHO - - 3, 5, 7, 9; 10% -   5.8 0.72
CD OptiCHO - - 3, 5, 7; 10% -   5.2 0.80
CD OptiCHO - - 3, 5, 7; 10% 3, 5, 7; 3.3%   6.3 1.74
CD OptiCHO daily; 3% daily; 0.3% - -   9.0 1.46
BalanCD CHO - - - - 1, 3, 5; 10% 7.1 1.30
  1. The time [d] for feed addition and the feed volume in % of the culture volume are indicated. Feed start for the culture in BalanCD CHO was day 1, all other cultures were fed from day 3 on. Values for peak cell concentration and harvest titer are mean values of triplicate experiments.