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Table 1 Proteins identified with at least 2 peptides and a false discovery rate of 0% in up to 5 biological replicates by MALDI-TOF/TOF-MS in the high molecular weight fractions 1 and 2.

From: Development of a chemically defined cultivation and transfection medium for HEK cell lines

High molecular weight fraction 1

High molecular weight fraction 2

Group

Protein name

# Peptides

Group

Protein name

# Peptides

Histones

Histone H2A

3

Histones

Histone H2A

4

 

Histone H2B

2

 

Histone H2B

3

 

Histone H4

2

 

Histone H4

4

 

Histone H3

3

   

Cytoskeleton

Tubulin alpha

2

   
 

Tubulin beta

2

Cytoskeleton

Tubulin alpha

2

 

Actin

3

 

Tubulin beta

3

 

Actin

6

   

Other

Galectin-3-binding protein

6

   
 

Heat shock 70 kDa protein 1A/1B

5

Extracellular (matrix)

Fibrillin-2

2

 

Fibronectin

5

   
    

Clusterin

3

    

Cochlin

2

   

Other

Galectin-3-binding protein

10

    

Heat shock 70 kDa protein 1A/1B

13

    

Golgi membrane protein 1

6

    

Alpha-enolase

2