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Table 1 ADCC assay systems

From: Cellular tools for biosimilar mAb analysis

Antibody Target cell line Read-out Selection of cell line
Trastuzumab HER-2+ SK-OV-3 cells metabolic activity of residual target cells selected from various breast cancer cell lines
Rituximab CD20+ Granta-519 cells Calcein release by target cells selected from various hematopoietic tumor cell lines
Cetuximab EGFR+ SK-OV-3 cells metabolic activity of residual target cells selected from various breast cancer cell lines
Infliximab membraneTNFalpha+ 293T cells Calcein release by target cells generated by genetic modification
Adalimumab membraneTNFalpha+ HT1080 cells metabolic activity of residual target cells