From: The successful transfer of a modern CHO fed-batch process to different single-use bioreactors
BIOSTAT® | RM 50 L | STR 50 L | B 5 L |
---|---|---|---|
Gassing principle | Overlay | Ring Sparger | |
Sensors | Single-use optical patches | Reusable probes | |
Working volume [L] | 25 | 50 | 5 |
Initial volume [L] | 13 | 26 | 2.6 |
pH set point | 7.15 | ||
pH control | CO2 gassing | ||
pO2 set point | 60% sat. | ||
pO2 control | Multi stage cascade comprising N2, Air, O2 - gassing | ||
Agitation [rpm] | 30 @ 10° rocking angle | 150 | 400 |