Table 1 Determination of the specific cell growth rate, specific production rate of lactate, ammonium and IFN4N, and specific consumption rate of glucose of CHO-K1 (a) and HEK 293T (b) clones.
From: Differences in the production of hyperglycosylated IFN alpha in CHO and HEK 293 cells
a) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Clones | μ(h-1) | qIFN (ng.10-6cell.h-1) | qgluc (μg.10-6cell.h-1) | qlac (μg.10-6cell.h-1) | qamon (nmol.10-6cell.h-1) | ||||||||||
P4D3 | 0,0182 | ± | 0,002 | 79 | ± | 8 | 36 | ± | 5 | 40 | ± | 5 | 0,027 | ± | 0,007 |
P1E9 | 0,0196 | ± | 0,002 | 35 | ± | 4 | 24 | ± | 4 | 45 | ± | 5 | 0,027 | ± | 0,003 |
P2A9 | 0,0249 | ± | 0,001 | 41 | ± | 4 | 30 | ± | 2 | 31 | ± | 1 | 0,014 | ± | 0,004 |
P1B6 | 0,0240 | ± | 0,002 | 19 | ± | 3 | 26 | ± | 5 | 49 | ± | 5 | 0,013 | ± | 0,005 |
P1B7 | 0,0191 | ± | 0,002 | 41 | ± | 3 | 32 | ± | 4 | 35 | ± | 2 | 0,017 | ± | 0,006 |
P1B8 | 0,0277 | ± | 0,002 | 42 | ± | 3 | 21 | ± | 3 | 34 | ± | 2 | 0,015 | ± | 0,004 |
b) | |||||||||||||||
Clones | μ(h -1 ) | q IFN (ng.10 -6 cell.h -1 ) | q gluc (μg.10 -6 cell.h -1 ) | q lac (μg.10 -6 cell.h -1 ) | q amon (nmol.10 -6 cell.h -1 ) | ||||||||||
P2A5 | 0,020 | ± | 0,001 | 129 | ± | 10 | 56 | ± | 6 | 37 | ± | 4 | 0,014 | ± | 0,003 |
P2C7 | 0,015 | ± | 0,002 | 122 | ± | 13 | 62 | ± | 11 | 38 | ± | 3 | 0,009 | ± | 0,003 |
P2G11 | 0,017 | ± | 0,002 | 82 | ± | 6 | 47 | ± | 9 | 31 | ± | 3 | 0,008 | ± | 0,002 |
P3B7 | 0,016 | ± | 0,002 | 99 | ± | 8 | 55 | ± | 8 | 31 | ± | 3 | 0,008 | ± | 0,003 |
P3H8 | 0,027 | ± | 0,001 | 82 | ± | 11 | 46 | ± | 6 | 34 | ± | 3 | 0,008 | ± | 0,001 |
P4B4 | 0,017 | ± | 0,002 | 63 | ± | 5 | 61 | ± | 15 | 32 | ± | 3 | 0,009 | ± | 0,001 |