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Table 1 Comparison of the glycosylation pattern of a model antibody produced in two different cell lines using the reference medium and the platform medium.

From: Development and evaluation of a new, specially tailored CHO media platform

Structure

Relative Amount of Glycan Structure [%]

 

High Producer 1

High Producer 2

 

Reference

Shaker

Platform

Shaker

Platform

Bioreactor

Reference

Shaker

Platform

Shaker

Platform

Bioreactor

Man3

-

-

-

4 ± 2

1 ± 2

-

Man5

7 ± 2

3 ± 2

5 ± 2

12 ± 3

9 ± 2

4 ± 1

G0F-GlcNAc

2 ± 2

2 ± 1

4 ± 2

10 ± 4

5 ± 0

2 ± 2

G0F

46 ± 2

50 ± 5

47 ± 5

47 ± 5

47 ± 2

47 ± 5

G1

6 ± 3

6 ± 2

8 ± 2

3 ± 1

9 ± 1

5 ± 1

G1F

30 ± 3

32 ± 5

29 ± 7

19 ± 2

23 ± 3

35 ± 2

G2F

9 ± 2

7 ± 2

7 ± 2

5 ± 1

6 ± 1

7 ± 2

  1. The relative amounts of detected structures are given in % with the standard deviation of four independent analyses.