Table 1 Determination of aeration and agitation strategy in the 80 L bioreactor, based on the maximum OTF required by the cells at 2 L and 10 L scales.
From: Scale-up considerations for monoclonal antibody production process: an oxygen transfer flux approach
| Â | 2 L | 10 L | Â | 80 L |
|---|---|---|---|---|
P/V [W.m -3 ] | 30 | 69 | Â | 80 |
Vs [×10 -4 m.s -1 ] | 0.94 | 3.53 |  | 4.03 |
k L a [×10 -3 s -1 ] | 0.70 | 1.43 |  | 3.85 |
%O 2 in gas mix | 74 | 90 | Â | 30 |
OTF max [×10 -3 s -1 ] | 2.44 | 6.11 | Target OTF for 80 L = 10 L OTF → | 5.55 |