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Figure 1 | BMC Proceedings

Figure 1

From: Characterization and quantitation of fluorescent Gag virus-like particles

Figure 1

Characterization of the purified standard Gag-GFP VLP material. (A) Confocal fluorescence microscopy image of a HEK 293 producer cell expressing green fluorescent Gag-GFP molecules. The lipid membrane is stained with Cell Mask™ (red) and the cell nucleus with Hoechst (blue). (B) TEM image of an ultrathin section showing VLP budding from HEK 293 producer cells. (C) Negatively stained Gag-GFP VLPs in the purified standard material. (D) Size exclusion chromatogram of the standard Gag-GFP VLP material. (E) SDS-PAGE and Western-blot analyses of the standard VLP material. Full and empty arrows represent Gag-GFP protein and Gag-GFP fragment, respectively. Abbreviations: MW, molecular weight standard.

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