Table 1 Bioreactor configuration and applications
From: A modular flow-chamber bioreactor concept as a tool for continuous 2D- and 3D-cell culture
Bioreactor design | Potential applications | Example | |
|---|---|---|---|
I. | Single channel, 6 variable culture inserts for 3D scaffolds transparent cover plate active aeration | Long term cultivation of 3D tissue constructs under flow conditions, tissue cultivation on implantable biomaterials | Cultivation of cartilage-carrier constructs [2] |
II. | 4 flow channels for perfusion 24 well plate layout inserts for 3D scaffolds surface aeration gas supply from humidified incubator | Simultaneous cultivation of four 3D constructs per channel, 4 channels available, separate functional tests can be carried out on single constructs | 3D cultures of liver cells [3], biomaterial testing |
III. | As (II), transparent bottom plate for microscopy flow channels instead of separate wells | Cultivation of shear-responsive cells, integration of biomaterials possible (e.g. a collagen membrane) | Cultivation of sweat-gland associated cells (current) |
IV. | As (II) plus integrated of electrodes for electrical stimulation and impedance measurement | Electrical stimulation of cell growth and orientation, impedance measurement of cell viability | Orientation of mitotic axis [5] |