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Table 1 PLS results for selected dye concentrations used in the fed-batch fermentation experiment.

From: 2D fluorescence spectroscopy for real-time aggregation monitoring in upstream processing

Dye

PC's

 

R-Square

RMSE

Offset

Slope

w/o dye

3

calibration data set

0.96

1.27

0.43

0.96

  

validation data set

0.72

3.62

2.75

0.70

2μM Bis-ANS

4

calibration data set

0.98

10.9

2.28

0.98

  

validation data set

0.93

19.36

-4.20

0.97

80μM ANS

4

calibration data set

0.98

0.82

0.18

0.98

  

validation data set

0.85

2.08

1.44

0.85

25μM Th T

2

calibration data set

0.99

5.18

0.52

0.99

  

validation data set

0.96

14.54

6.23

0.93

  1. 2D fluorescence scans were taken as x-data and the mAb aggregate concentration was used as target data for chemometric modeling. Validation data sets were generated with cross validation.