Table 2
From: Use of microcarriers in Mobius® CellReady bioreactors to support growth of adherent cells
MDCK/ Cytodex 3 ® Microcarriers Process Table | ||
|---|---|---|
Variable | Value | |
Cells | MDCK | hMSCs |
Inoculation Density | 4e5 cells/mL | 5e3 cells/mL |
Substrate | Cytodex 3 ® | Solohill® |
Growth Media | DMEM w/ 4.5g/L Glucose, 2% FBS, 1% NEAA and 2mM L-Glutamine | DMEM low glucose, 10% FBS, 8ng/ml bFGF, 2mM Glutamine, 1X Pen/Strep |
pH | 7 | NA |
DO (% Saturation) | 45 | NA |
Feed 1 | Day 1: 100% Growth Media | Day 6: 1000ml low glucose fresh medium |
Feed 2 | Day 3: Drain 50% of the working volume and reefed with equal volume of Growth Media | Day 9: 400ml high glucose fresh medium |
Batch Duration | 7 days | 12 days |