From: CHO starter cell lines for manufacturing of proteins with pre-defined glycoprofiles
Model protein: human IgG | Model protein: Fc-Fusion protein | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Clone pool no. | 1 | 2 | 4 | 6 | 7 | 10 | ||||||||
Index | A | B | A | B | A | B | A | B | A | B | A | B | ||
relative modulator mRNA expression | ||||||||||||||
GM2 | 5.8 | 5.8 | 2.5 | 2.5 | 0.4 | 0.4 | 1.5 | 1.5 | 3.7 | 3.7 | 0.6 | 0.6 | ||
GM3 | 3.2 | 3.2 | 1.4 | 1.4 | 0.3 | 0.3 | ||||||||
Key process parameter | ||||||||||||||
Peak VCD | (cell/mL) | 20 | 20 | 31 | 24 | 28 | 24 | 31 | 24 | 29 | 25 | 27 | 25 | |
Final-vitality | (%) | 73 | 82 | 82 | 88 | 87 | 91 | 87 | 87 | 86 | 84 | 89 | 93 | |
Titer | (g/L) | 0.6 | 0.4 | 0.9 | 0.7 | 1.0 | 0.6 | 2.1 | 1.0 | 1.8 | 1.0 | 2.3 | 1.1 | |
N-Glycan analysis | ||||||||||||||
G0F | (%) | 2 | 1 | 62 | 55 | 71 | 68 | 37 | 24 | 19 | 19 | 46 | 41 | |
G1F | (%) | 19 | 13 | 23 | 29 | 18 | 21 | 27 | 32 | 35 | 34 | 33 | 38 | |
G2 | (%) | 3 | 4 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
G2F | (%) | 61 | 67 | 4 | 6 | 2 | 3 | 3 | 7 | 11 | 10 | 9 | 12 | |
G1FS1 | (%) | 2 | 3 | <1 | <1 | <1 | <1 | 9 | 9 | 5 | 7 | 0 | 0 | |
G2FS1 | (%) | 2 | 2 | <1 | <1 | <1 | <1 | 19 | 21 | 22 | 11 | 1 | 9 |