Figure 1

A map associating the differentially expressed amino acid transporters for the null cell line, their amino acid substrates, and the intracellular concentrations (femtomol/ cell, in the area designated by the "IN" tag) and extracellular concentrations (mM, in the area designated by the "OUT" tag) of the latter. A.a. transport is highlighted by the black box. The expression of the mRNA levels of the differentially expressed a.a. transporters (in mRNA copies per cell) at different phases of cell culture, exponential (day 4), stationary (days 6 & 7), and decline (day 9) is displayed at the bottom, where stationary phase samples are averaged, since not statistically different (for ease of statistical analysis visualization). The relevant energy utilisation mechanisms of each system are also depicted (top). Genes: slc6a9 (glycine), slc1a2 (acidic a.a.), slc7a7 (basic and branched chain a.a.) and its heteromeric transporter slc3a2 were also found to be differentially expressed, but are not presented in this figure. Our chosen a.a. analysis method was not able to quantify cysteine (L-Cys) levels.