Table 1 Culture Parameter Conditions and Set-Points
From: Optimized platform medium and feed for rCHO cell lines using the CHEF1® expression system
Parameters | ||
|---|---|---|
Shake Flask/Culture Volume | 125 mL vented Erlenmeyer | 30 mL working volume |
Bioreactor/Culture Volume | 3 L single-use CellReady bioreactor | 2 L working volume |
Seeding Density | 5x105 viable cells/ mL | |
Temperature | 37°C ± 0.5°C (days 0 - 4) | 34°C ± 0.5°C (day 5 - end) |
CO 2 Level (Shake Flask) | 6% ± 1% (days 0 - 4) | 2% ± 1% (day 5 - end) |
pH (Bioreactor) | 7.0 ± 0.2 | |
RPM (Shake Flask) | 120 ± 5 | |
RPM (Bioreactor) | 200 ± 10 | |
Dissolved Oxygen (Bioreactor) | 60% ± 5% | |