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Figure 1 | BMC Proceedings

Figure 1

From: Profiling of glycosylation gene expression in CHO fed-batch cultures in response to glycosylation-enhancing medium components

Figure 1

Glycan analysis data measured as the percentage of total glycans. (A) The glycan profile for the CHO-S® culture with no addition of components A and/or B. (B) These data indicate that the addition of component A to the culture results in very little change to the glycan profile, only slight increase in the percent of G1F on days 5 and 7. (C) The addition of component B to the culture shifts the glycan profile from primarily non-galactosylated G0F to increased G1F (single galactose) and G2F (two galactose) glycoforms. (D) The addition of both components A and B results in a change in galactosylation indicated by the increase in both G1F and G2F and an overall reduction of G0F. In every condition, G0F increases with time but this is minimized with the addition of both components A and B. The majority of protein glycoforms within this experiment are fucosylated and the addition of components A and/or B does not appear to alter this.

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BMC Proceedings

ISSN: 1753-6561

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