Table 1 Universal Primers and their Variants [16, 17]

U8F

AGAGTTTGATCCTGGCTCAG

1

4

51.78→51.78

0.50→0.50

20

1553

U336R

ACTGCTGCSYCCCGTAGGAGTCT

4

4

60.62→62.40

0.61→0.65

22

7408

U337F

GACTCCTACGGGAGGCWGCAG

2

6

60.21→60.21

0.67→0.67

21

7689

U518R

GTATTACCGCGGCTGCTGG

1

6

55.41→55.41

0.63→0.63

18

8417

U533F

GTGCCAGCMGCCGCGGTAA

2

6

57.56→59.72

0.68→0.74

19

8929

U785F

GGATTAGATACCCTGGTA

1

4

45.77→45.77

0.44→0.44

18

8183

U805R

GACTACCAGGGTATCTAATC

1

4

49.73→49.73

0.45→0.45

20

8171

U907R

CCGTCAATTCCTTTRAGTTT

2

4

45.63→47.68

0.35→0.40

20

7296

U928F

TAAAACTYAAAKGAATTGACGGG

4

4

48.14→51.71

0.30→0.39

23

5090

U1100F

YAACGAGCGCAACCC

2

4

44.67→47.41

0.60→0.67

15

8980

U1492R

GGTTACCTTGTTACGACTT

1

3

46.77→46.77

0.42→0.42

19

449

HMPV1F

GAGTTTGATCCTGGCTCAG

1

4

51.09→51.09

0.53→0.53

20

1778

HMPV3R

ATTACCGCGGCTGCTGG

1

6

51.88→51.88

0.65→0.65

17

8428

HMPV3F

CCTACGGGAGGCAGCAG

1

3

54.29→54.29

0.71→0.71

17

8735

HMV5R

CCCGTCAATTCMTTTRAGT

4

4

44.62→48.93

0.37→0.47

19

8533

HMPV6F

GYAACGAGCGCAACCC

2

4

48.50→51.06

0.63→0.69

16

8507

HMPV9R

AAGGAGGTGATCCAGCCGCA

1

4

55.88→55.88

0.60→0.60

20

1193

N337F

GGAGGCAGCAGTRRGGAAT

4

2

51.09→55.41

0.53→0.63

19

8570

N775R

CTACCRGGGTATCTAATCC

2

4

48.93→51.09

0.47→0.53

19

8427

K331F

CCTACGGGNGGCWGCAG

8

6

54.29→56.70

0.71→0.76

17

8882

K775R

GACTACHVGGGTATCTAATCC

9

10

50.45→54.36

0.43→0.52

21

8868

S899R

CCGTCAATTYMTTTRAGT

8

4

38.93→45.77

0.28→0.44

18

8779