Table 4 Select Designed Primers
From: Better primer design for metagenomics applications by increasing taxonomic distinguishability
regionID | 5'→3' sequence | deg | sDimer | TmRange | CG%Range | nHits | posEcoli |
|---|---|---|---|---|---|---|---|
FV3 | ACWCCTRCGGGWGGCWG | 16 | 4 | 51.88→54.29 | 0.65→0.70 | 8867 | 345 |
FV3 | ACWCCTRCGGGWGGCWGCAG | 16 | 6 | 57.93→59.98 | 0.65→0.70 | 8854 | 345 |
FV4 | AGCAGCCGCGGTAANACG | 4 | 6 | 52.60→54.88 | 0.61→0.67 | 8305 | 528 |
FV6 | WACSCGMRGAACCTTACC | 16 | 6 | 48.04→52.60 | 0.50→0.61 | 8045 | 975 |
FV7 | ATGGYYGTCGTCARCTCG | 8 | 4 | 48.04→54.88 | 0.50→0.67 | 8821 | 1062 |
FV7 | AGTCCNRYAACGAGCGCAACC | 16 | 4 | 54.36→60.21 | 0.52→0.67 | 8788 | 1103 |
FV8 | AGGAAGGHGDGGAYGASGTC | 36 | 6 | 53.83→59.98 | 0.55→0.70 | 8810 | 1185 |
RV3 | TTACCGCGGCTGCTGGCAC | 1 | 6 | 57.56→57.56 | 0.68→0.68 | 8926 | 505 |
RV3 | TWTYACCGCGGCTGCTGG | 4 | 6 | 52.60→54.88 | 0.61→0.67 | 8543 | 508 |
RV4 | ACCAGGGTATCTAAKCCTG | 2 | 4 | 48.93→51.09 | 0.47→0.53 | 8292 | 773 |
RV6 | TYACRRCACGAGCTGWCG | 16 | 5 | 48.04→54.88 | 0.50→0.67 | 9026 | 1052 |
RV7 | ACGTCRTCCHCWCCTTCC | 12 | 4 | 50.32→54.88 | 0.56→0.67 | 8114 | 1166 |
RV8 | TGWGTACAAGRYCCGRGAACG | 16 | 6 | 52.40→58.26 | 0.48→0.62 | 8411 | 1367 |