Mapping of conservation information onto the sequence, structure, and network representations. The nodes and residues in the RIN (top left) and chain A of scTIM (top right) are colored according to the conservation scores retrieved from Consurf-DB (turquoise-to-pink coloring indicates variable-to-conserved sites). The network nodes that represent mutated residues with a high conservation score (F11, L13, Q82, I83, I109, K134, K135, L174, A175, D180, A212, N213, V226) are selected using two filters in Cytoscape (left) and highlighted in the network view by yellow color (top left) and in the other two views by green boundary around the structure (top right) or the amino acid letter (bottom right). Nodes that correspond to mutated residues are depicted as diamonds. Additional data annotated to the residue nodes is shown in the Cytoscape attribute browser as table (bottom left).